Immobilization of Chondroitin Sulfate A onto Monolithic Epoxy Silica Column as a New Chiral Stationary Phase for High-Performance Liquid Chromatographic Enantioseparation

Chondroitin sulfate A was covalently immobilized onto a monolithic silica epoxy column involving Schiff base formation in the presence of ethylenediamine as spacer and evaluated terms its selectivity enantioseparation. The obtained utilized chiral stationary phase enantioseparation amlodipine verapamil using mobile consisting 50 mM phosphate buffer pH 3.5 UV detection. Sample dilution by organic solvents (preferably 25% v/v acetonitrile-aqueous solution) applied to achieve baseline enantioresolution (Rs > 3.0) individual drug models within 7 min, an excellent linearity (R2 = 0.999) interday repeatability 1.1% 1.8% RSD. performance for quantification racemate commercial tablets showed recovery 86–98% from tablet matrices. Computational modeling molecular docking employed investigate feasible complexes between enantiomers selector.